hfob cells procell Search Results


hfob cells procell  (Procell Inc)
90
Procell Inc hfob cells procell
Toggling between normal and transformed growth based on sensor proteins. a Reductions in sensor protein expression levels in transformed cells (HOS and 143B) compared to nontransformed cells <t>(hFOB,</t> U2OS, and MG63), as determined by quantitative proteomics. n = 3 in each group; the data are presented as the means. Representative Western blots of sensor proteins (TPM1, TPM2, and TPM3) in U2OS and 143B cells ( b ) and quantification ( c ). n = 3 in each group; the data are presented as the mean ± s.d. values. Staining for paxillin (green) in TPM1 - and TPM2 -silenced U2OS cells and TPM3 -silenced 143B <t>cells</t> <t>cultured</t> on glass surfaces overnight ( d ) and quantification ( e , f ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Staining for actin (red) in TPM1 - or TPM2 -silenced U2OS cells and TPM3 -silenced 143B cells cultured on rigid (40 kPa) and soft (4 kPa) hydrogel surfaces overnight ( g ) and quantification ( h , i ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Soft agar cultures of TPM3 -silenced 143B cells for 2 weeks ( j ) and quantification ( k , l ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Sphere cultures of TPM3 -silenced 143B cells propagated for 7 days ( m ) and quantification ( n ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. The graphs show the individual data points derived from three independent measurements and the means. * P < 0.05; ** P < 0.01; **** P < 0.000 1. Scale bars, 50 μm
Hfob Cells Procell, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hfob cells procell/product/Procell Inc
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hfob cells procell - by Bioz Stars, 2026-03
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hfob cells cl-0353  (Procell Inc)
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Procell Inc hfob cells cl-0353
A, Western blot assay of COLEC12 expression in tumor and para‐tumor tissue. B, Western blot assay of COLEC12 expression in <t>hFOB,</t> <t>143B,</t> MG‐63, Saos‐2, and HOS cells. C, Quantification. and D, Quantitative RT‐PCR assay for mRNA level of COLEC12 in different tissues. E, Quantification. and F, Quantitative RT‐PCR assay for mRNA level of COLEC12 in different cells. Protein and mRNA levels were normalized to β‐actin. (tumor or Saos‐2 cells vs. para‐tumor or hFOB cells, * P < .05, n = 6 per group; all data were represented as mean ± standard error)
Hfob Cells Cl 0353, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hfob cells cl-0353/product/Procell Inc
Average 90 stars, based on 1 article reviews
hfob cells cl-0353 - by Bioz Stars, 2026-03
90/100 stars
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Toggling between normal and transformed growth based on sensor proteins. a Reductions in sensor protein expression levels in transformed cells (HOS and 143B) compared to nontransformed cells (hFOB, U2OS, and MG63), as determined by quantitative proteomics. n = 3 in each group; the data are presented as the means. Representative Western blots of sensor proteins (TPM1, TPM2, and TPM3) in U2OS and 143B cells ( b ) and quantification ( c ). n = 3 in each group; the data are presented as the mean ± s.d. values. Staining for paxillin (green) in TPM1 - and TPM2 -silenced U2OS cells and TPM3 -silenced 143B cells cultured on glass surfaces overnight ( d ) and quantification ( e , f ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Staining for actin (red) in TPM1 - or TPM2 -silenced U2OS cells and TPM3 -silenced 143B cells cultured on rigid (40 kPa) and soft (4 kPa) hydrogel surfaces overnight ( g ) and quantification ( h , i ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Soft agar cultures of TPM3 -silenced 143B cells for 2 weeks ( j ) and quantification ( k , l ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Sphere cultures of TPM3 -silenced 143B cells propagated for 7 days ( m ) and quantification ( n ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. The graphs show the individual data points derived from three independent measurements and the means. * P < 0.05; ** P < 0.01; **** P < 0.000 1. Scale bars, 50 μm

Journal: Bone Research

Article Title: Impairment of rigidity sensing caused by mutant TP53 gain of function in osteosarcoma

doi: 10.1038/s41413-023-00265-w

Figure Lengend Snippet: Toggling between normal and transformed growth based on sensor proteins. a Reductions in sensor protein expression levels in transformed cells (HOS and 143B) compared to nontransformed cells (hFOB, U2OS, and MG63), as determined by quantitative proteomics. n = 3 in each group; the data are presented as the means. Representative Western blots of sensor proteins (TPM1, TPM2, and TPM3) in U2OS and 143B cells ( b ) and quantification ( c ). n = 3 in each group; the data are presented as the mean ± s.d. values. Staining for paxillin (green) in TPM1 - and TPM2 -silenced U2OS cells and TPM3 -silenced 143B cells cultured on glass surfaces overnight ( d ) and quantification ( e , f ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Staining for actin (red) in TPM1 - or TPM2 -silenced U2OS cells and TPM3 -silenced 143B cells cultured on rigid (40 kPa) and soft (4 kPa) hydrogel surfaces overnight ( g ) and quantification ( h , i ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Soft agar cultures of TPM3 -silenced 143B cells for 2 weeks ( j ) and quantification ( k , l ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Sphere cultures of TPM3 -silenced 143B cells propagated for 7 days ( m ) and quantification ( n ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. The graphs show the individual data points derived from three independent measurements and the means. * P < 0.05; ** P < 0.01; **** P < 0.000 1. Scale bars, 50 μm

Article Snippet: hFOB cells (Procell) were cultured at 34 °C in a 5% CO 2 incubator in Dulbecco’s modified Eagle’s medium/F12 supplemented with 10% fetal bovine serum (FBS).

Techniques: Transformation Assay, Expressing, Quantitative Proteomics, Western Blot, Staining, Cell Culture, Derivative Assay

A, Western blot assay of COLEC12 expression in tumor and para‐tumor tissue. B, Western blot assay of COLEC12 expression in hFOB, 143B, MG‐63, Saos‐2, and HOS cells. C, Quantification. and D, Quantitative RT‐PCR assay for mRNA level of COLEC12 in different tissues. E, Quantification. and F, Quantitative RT‐PCR assay for mRNA level of COLEC12 in different cells. Protein and mRNA levels were normalized to β‐actin. (tumor or Saos‐2 cells vs. para‐tumor or hFOB cells, * P < .05, n = 6 per group; all data were represented as mean ± standard error)

Journal: Journal of Clinical Laboratory Analysis

Article Title: COLEC12 regulates apoptosis of osteosarcoma through Toll‐like receptor 4–activated inflammation

doi: 10.1002/jcla.23469

Figure Lengend Snippet: A, Western blot assay of COLEC12 expression in tumor and para‐tumor tissue. B, Western blot assay of COLEC12 expression in hFOB, 143B, MG‐63, Saos‐2, and HOS cells. C, Quantification. and D, Quantitative RT‐PCR assay for mRNA level of COLEC12 in different tissues. E, Quantification. and F, Quantitative RT‐PCR assay for mRNA level of COLEC12 in different cells. Protein and mRNA levels were normalized to β‐actin. (tumor or Saos‐2 cells vs. para‐tumor or hFOB cells, * P < .05, n = 6 per group; all data were represented as mean ± standard error)

Article Snippet: The following reagents and antibodies were used in this study: hFOB cells (CL‐0353, Procell), 143B cells (CRL‐8303, ATCC), MG‐63 cells (CL‐0157, Procell), Saos‐2 cells (CL‐0202, Procell), and HOS cells (CL‐0360, Procell).

Techniques: Western Blot, Expressing, Quantitative RT-PCR